banner

Tissue Digestion - Technical Information

> Composition of Mammals
> Digestion of Radioiodine Labeled Tissues
> Efficacy Testing Results
> Formaldehyde Destruction
> Prion Destruction
> The Effect on Plant and Animal Tissues
> Water Usage

Efficacy Testing Results

eff

Efficacy Testing and Discussion
Refer to the "WR2 Process Efficacy Testing Results" table in the Brochure. WR2 Process efficacy is due to the high temperature and duration of the process, which exposes all materials within the Tissue Digestor to a temperature of 300 degrees Fahrenheit for a standard 3 hours exposure time. The exposure is amplified by the fact that it is a wet, continuously agitated, process eliminating any possibility of cold spots. The thermal transfer properties of this wet system are substantially better than in a steam sterilization system. There are no known pathogens that can survive exposure to this time and temperature profile. In addition, all proteins, including prions, are destroyed by Alkaline Hydrolysis in the WR2 Process.

Agents listed under the DHHS Final Rule
Additional Requirements for Facilities Transferring or Receiving Select Agents

Bacterial Rickettsiae Fungi
Bacillus anthracis
Burkholderia (Pseudomanas) mallei Clostridium botulinum Yersinia pestis
Brucella abortus, B. melitensis, B. suis Burkholderia (Pseudomonas) pseudomallei
Francisella tularensis
Coxiella burnetti
Rickettsia rickettsii
Rickettsia prowazekii
Coccidioides immitis

The above bacteria, rickettsiae, and fungi would be destroyed in the same manner as the indicator organisms shown in the report to New York State

Viruses Toxins

Crimean-Congo Hemorrhagic Fever virus

South American Hemorrhagic Fever virus (Junin, Machupo, Sabia, Flexal Guanarito)

Eastern Equine Encephalitis virusTick-borne Encephalitis complex viruses

Viruses causing Hantavirus Pulmonary Syndrome

Variola major virus (Smallpox virus)

Venezuelan Equine Encephalitis virus

Ebola virus

Lassa Fever virus

Equine Morbillivirus

Marburg virus

Yellow Fever virus

Rift Valley Fever virus

Abrin

Aflatoxins

Botulinum toxins

Clostridium perfringens

Epsilon toxin

Conotoxins

Diacetoxyscirpenol

Ricin

Saxitoxin

Shigatoxin

Staphylococcal enterotoxin

Tetrodotoxin

T-2 toxin

Protein toxins would be hydrolyzed in the same manner as any other protein
Nonprotein toxins would be destroyed as shown in subsequent pages

Recombinant Organisms/Molecules
Recombinant organisms and molecules shown to produce or encode for a factor associated with a disease would be destroyed in the same manner as natural organisms

Recombinant organisms or nucleic acid sequences coding for any of the toxins listed above or their toxic subunits would be destroyed in the same manner as normal nucleic acids and nucleotides